David Worthylake PhD

Associate Professor

Department of Biochemistry & Molecular Biology
1901 Perdido St., Box P7-2
New Orleans, LA 70112
Phone: 504-568-5176

dworth@lsuhsc.edu

Click here for a link to ongoing research in this laboratory.

From 1998 to 2004, Dr. Worthylake worked with Dr. John Sondek at the University of North Carolina at Chapel Hill studying the mechanism of activation of Rho GTPases by members of the Dbl family of guanine nucleotide exchange factors. His lab is currently studying the functioning of the IQGAP family of large molecular scaffolds, and in a collaboration with the laboratories of Dr. Sunyoung Kim and Dr. Edward Wojcik, the allosteric regulation of the mitotic kinesin motor proteins.

Allosteric drug discrimination is coupled to mechanochemical changes in the Kinesin-5 motor core.Kim ED, Buckley RS, Learman S, Richard J, Parke C, Worthylake DK, Wojcik EJ, Walker RA, Kim S.J Biol Chem. Mar 18. (2010)

ATP hydrolysis in Eg5 kinesin involves a catalytic two-water mechanism.Parke CL, Wojcik EJ, Kim S, Worthylake DK.J Biol Chem. Dec 15 (2009).

Regulation of ROCKII by localization to membrane compartments and binding to DynaminI.Tumusiime S, Rana MK, Kher SS, Kurella VB, Williams KA, Guidry JJ, Worthylake DK, Worthylake RA.Biochem Biophys Res Commun. 2009 Apr 10;381(3):393-6. Feb 15. (2009)

Crystal structure of the GAP-related domain from IQGAP1.Kurella VB, Richard JM, Parke CL, Lecour LF Jr, Bellamy HD, Worthylake DK. J Biol Chem. Mar 25. (2009)

Tumusiime S, Rana MK, Kher S, Kurella VB, Williams KA, Guidry JJ, Worthylake DK, Worthylake RA.Regulation of ROCKII by localization to membrane compartments and binding to DynaminI.Biochem Biophys Res Commun. Feb 15. (2009)

Worthylake DK, Rossman KL, Sondek J, Crystal Structure of the DH/PH fragment of Dbs without bound GTPase, Structure 12(6):1079-1086, 2004.

Worthylake DK, Rossman KL, , Snyder JT, Cheng L, Whitehead IP, Sondek J, Functional analysis of Cdc42 residues required for GEF-catalyzed exchange, J Biol Chem 277(52):50893-8, 2002.

Worthylake DK, Rossman KL & Sondek J, Crystal structure of Rac1 in complex with the guanine nucleotide exchange region of Tiam1, Nature 408(6813):682-8, 2000.

Gamble TR, Yoo S, Vajdos FF, von Schwedler UK, Worthylake DK, Wang H, McCutcheon JP, Sundquist WI, Hill CP, Structure of the Carboxyl-Terminal Dimerization Domain of the HIV-1 Capsid Protein, Science 278 849 – 853, 1997.

Hill CP, Worthylake D, Bancroft DP, Christensen AM, Sundquist WI, Crystal structures of the trimeric human immunodeficiency virus type 1 matrix protein: Implications for membrane association and assembly, Proc Natl Acad Sci 93 3099 – 3104, 1996.

To do a search for all of Dr. Worthylake's publications click here.