William C. Claycomb, PhD

Professor, Biochemistry

Department of Biochemistry & Molecular Biology
1901 Perdido St., Box P7-2
Medical Education Building Room 7238
New Orleans, LA 70112
Phone: (504) 568-4737
Fax: (504) 568-7649

wclayc@lsuhsc.edu

Click here for a link to ongoing research in this laboratory.

Degrees

PhD - 1969
Indiana University

Bio Dr. Claycomb obtained his Bachelor of Science in Zoology from Indiana University in 1966. His Doctorate was awarded in 1969 from the Indiana University School of Medicine. From 1969 to 1972, Dr. Claycomb was a Research Associate at the Harvard Medical School in Biological Chemistry in Boston where he was a NIH postdoctoral fellow. From 1972 to 1976 he was an Assistant Professor at the Baylor College of Medicine in Houston. Dr. Claycomb then joined the faculty at LSUHSC as an Associate Professor and was an Established Investigator of the American Heart Association. He currently is Professor of Biochemistry and Molecular Biology at LSUHSC.
Research Interests Biochemistry of cell differentiation
Biochemistry of cell proliferation
Cell cycle control
Selected Publications

Hashem SI, Lam ML, Mihardja SS, White SM, Lee RJ, Claycomb WC.Shox2 Regulates the Pacemaker Gene Program in Embryoid Bodies. Stem Cells Dev. 2013 Jul 26. Open PDF of this article

Hashem SI, Claycomb WC.Genetic isolation of stem cell-derived pacemaker-nodal cardiac myocytes. Mol Cell Biochem. 2013 Jul 23. [Epub ahead of print] PMID: 23877224 Open PDF of this article

Fatima NSchooley JF JrClaycomb WCFlagg TP. Promoter DNA methylation regulates murine SUR1 (Abcc8) and SUR2 (Abcc9) expression in HL-1 cardiomyocytes. PLoS One. 2012;7(7):e41533. Epub 2012 Jul 23

Touchberry CD, Orange M, Weisleder N, Brotto M, Claycomb WC, Wacker MJ.Store-operated calcium entry is present in HL-1 cardiomyocytes and contributes to resting calcium.Biochem Biophys Res Commun.(2011) Nov 6. [Epub ahead of print]

Pieperhoff S, Rickelt S, Heid H, Claycomb W C, Kuhn C, Frey N, Franke WW.The plaque protein myozap identified as a novel major component of adhering junctions in endothelia of the blood and the lymph vascular systems.J Cell Mol Med. (2011) Oct 13.

Vinciguerra M, Santini MP, Claycomb WC, Ladurner AG and Rosenthal N.mIGF-1/JNK1/SirT1 signaling confers protection against oxidative stress in the heart. Aging Cell. (2011) In Press.

Lam ML, Hashem SI, Claycomb WC. Embryonic stem cell-derived cardiomyocytes harbor a subpopulation of niche-forming Sca-1+ progenitor cells. Molecular Cellular Biochemistry, 349:69-76 (2011)
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Ma X, Jana SS, Conti MA, Kawamoto S, Claycomb WC, Adelstein RS.Ablation of Nonmuscle Myosin II-B and II-C Reveals a Role for Nonmuscle Myosin II in Cardiac Myocyte Karyokinesis. Moleular Biology of the Cell. (2011)21:3952-3962.
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Dong C, Yang L, Zhang X, Gu H, Lam ML, Claycomb WC, Xia H, Wu G. Rab8 Interacts with Distinct Motifs in α2B- and β2 -Adrenergic Receptors and Differentially Modulates Their Transport. J. Biol. Chem. 2010; 285: 20369-20380. Open PDF of this article

Vinciguerra M, Santini MP, Claycomb WC, Ladurner AG, Rosenthal N. Local IGF-1 isoform protects cardiomyocytes from hypertrophic and oxidative stresses via SirT1 activity. Aging (Albany NY). 2010; 2:43-62.
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Ikeda K, Tojo K, Inada Y, Takada Y, Sakamoto M, Lam M, Claycomb WC, Tajima N. Regulation of urocortin I and its related peptide urocortin II by inflammatory and oxidative stresses in HL-1 cardiomyocytes. J Mol Endocrinol. 2009; 42:479-89.
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Brunt KR, Tsuji MR, Lai JH, Kinobe RT, Durante W, Claycomb WC, Ward C, Melo LG .Heme Oxygenase-1 Inhibits Pro-Oxidant Induced Hypertrophy In HL-1 Cardiomyocytes.Exp Biol Med (Maywood). Feb 25.(2009)
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Shimko VF, Claycomb WC, Effect of Mechanical Loading on Three-Dimensional Cultures of Embryonic Stem Cell-Derived Cardiomyocytes.Tissue Eng Part A. 2008; 14:49-58.
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Landstrom AP, Weisleder N, Batalden KB, Bos JM, Tester DJ, Ommen SR, Wehrens XH, Claycomb WC, Ko JK, Hwang M, Pan Z, Ma J, Ackerman MJ. Mutations in JPH2-encoded junctophilin-2 associated with hypertrophic cardiomyopathy in humans. J Mol Cell Cardiol. 2007; 42:1026-35.
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George CH, Rogers SA, Bertrand BM, Tunwell RE, Thomas NL, Steele DS, Cox EV, Pepper C, Hazeel CJ, Claycomb WC, Lai FA. Alternative splicing of ryanodine receptors modulates cardiomyocyte Ca2+ signaling and susceptibility to apoptosis. Circ Res. 2007; 100:874-83.
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Hidalgo-Bastida LA, Barry JJ, Everitt NM, Rose FR, Buttery LD, Hall IP, Claycomb WC, Shakesheff KM. Cell adhesion and mechanical properties of a flexible scaffold for cardiac tissue engineering. Acta Biomater. 2007; 3:457-62.
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White SM, Claycomb WC. Embryonic stem cells form an organized, functional cardiac conduction system in vitro. Am J Physiol Heart Circ Physiol. Feb.;288(2):H670-9 (2005)
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Barnes K, Dobrzynski H, Foppolo S, Beal PR, Ismat F, Scullion ER, Sun L, Tellez J, Ritzel MW, Claycomb WC, Cass CE, Young JD, Billeter-Clark R, Boyett MR, Baldwin SA. Distribution and functional characterization of equilibrative nucleoside transporter-4, a novel cardiac adenosine transporter activated at acidic pH. Circ Res. 2006; 99:510-9.
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Filipeanu CM, Zhou F, Lam ML, Kerut KE, Claycomb WC, Wu G. Enhancement of the recycling and activation of beta-adrenergic receptor by Rab4 GTPase in cardiac myocytes. J Biol Chem., Apr 21;281(16):11097-103 (2006)
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Ikeda K, Tojo K, Otsubo C, Udagawa T, Kumazawa K, Ishikawa M, Tokudome G,Hosoya T, Tajima N, Claycomb WC, Nakao K, Kawamura M. 5-hydroxytryptamine synthesis in HL-1 cells and neonatal rat cardiocytes. Biochem Biophys Res Commun. Mar. 11;328(2):522-5 (2005) Open PDF of this article

Chandrasekar B, Mummidi SM, Claycomb WC, Mestril R, Nemer MD. Interleukin-18 is a pro-hypertrophic cytokine that acts through a phosphatidylinositol 3-kinase- phosphoinositide-dependent kinase-1-Akt-GATA4 signaling pathway in cardiomyocytes. J Biol Chem. Dec 1; (2004)
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Filipeanu CM, Zhou F, Claycomb WC, Wu G. Regulation of the cell-surface expression and function of angiotensin II type 1 receptor by Rab1-mediated ER-to-Golgi transport in cardiac myocytes. J Biol Chem. Sep. 24;279(39):41077-84 (2004)
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White SM, Constantin PE, Claycomb WC. Cardiac physiology at the cellular level: use of cultured HL-1 cardiomyocytes for studies of cardiac muscle cell structure and function. Am J Physiol Heart Circ Physiol. 2004; 286:H823-9.
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Seymour EM, Wu SY, Kovach MA, Romano MA, Traynor JR, Claycomb WC, Bolling SF. HL-1 myocytes exhibit PKC and K(ATP) channel-dependent delta opioid preconditioning. J Surg Res.Oct;114(2):187-94 (2003)
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White SM, Claycomb WC. Cardiac cell transplantation: protocols and applications. Methods Mol Biol., ;219:83-95. (2003) No abstract or PDF available

Lam ML, Bartoli M, Claycomb WC. The 21-day postnatal rat ventricular cardiac muscle cell in culture as an experimental model to study adult cardiomyocyte gene expression. Mol Cell Biochem., Jan.;229(1-2):51-62. (2002)
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Neilan CL, Kenyon E, Kovach MA, Bowden K, Claycomb WC, Traynor JR, Bolling SF. An immortalized myocyte cell line, HL-1, expresses a functional delta –opioid receptor. J Mol Cell Cardiol., Dec;32(12):2187-93. (2000)
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Lanson NA Jr, Egeland DB, Royals BA, Claycomb WC. The MRE11-NBS1-RAD50 pathway is perturbed in SV40 large T antigen-immortalized AT-1, AT-2 and HL-1 cardiomyocytes. Nucleic Acids Res., Aug. 1;28(15):2882-92.(2000)
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Nguyen SV, Claycomb WC. Hypoxia regulates the expression of the adrenomedullin and HIF-1 genes in cultured HL-1 cardiomyocytes.Biochem Biophys Res Commun. Nov 19;265(2):382-6. (1999)
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Cormier-Regard S, Nguyen SV, Claycomb WC. Adrenomedullin gene expression is developmentally regulated and induced byhypoxia in rat ventricular cardiac myocytes. J Biol Chem., Jul. 10;273(28):17787-92. (1998)
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Watanabe E, Smith DM Jr, Delcarpio JB, Sun J, Smart FW, Van Meter CH Jr, Claycomb WC. Cardiomyocyte transplantation in a porcine myocardial infarction model. Cell Transplant, May-Jun.;7(3):239-46. (1998) View Abstract

Claycomb WC, Lanson NA Jr, Stallworth BS, Egeland DB, Delcarpio JB, Bahinski A, Izzo NJ Jr. HL-1 cells: a cardiac muscle cell line that contracts and retains phenotypic characteristics of the adult cardiomyocyte. Proc Natl Acad Sci U S A., 1998 Mar. 17;95(6):2979-84.
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