haas_arthur

Arthur Haas, PhD

Roland Coulson Professor and Chairman
 

Department of Biochemistry & Molecular Biology
1901 Perdido Street, Box P7-2
New Orleans, LA 70112
504.568.3004
Office MEB 7103 A
Fax # 504.568.2093
ahaas@lsuhsc.edu

Click here for a link to ongoing research in this laboratory.

Degrees

PhD - 1979
Northwestern University, Chicago, IL

Bio

Dr. Haas obtained his Bachelor of Science in Biochemistry from Texas Christian University in 1974. His Doctorate in Biochemistry was awarded in 1979 by Northwestern University School of Medicine in Chicago. From 1979 to 1983, Dr. Haas was on the staff of the Institute for Cancer Research of the Fox Chase Cancer Center in Philadelphia where he was a NIH postdoctoral fellow. During this time he co-discovered the role of ubiquitin in protein turnover with Irwin Rose, Avram Hershko, and Aaron Ciechanover, who shared the 2004 Nobel Prize in Chemistry for this work. Since 1983, he has been a faculty member in the Department of Biochemistry at the Medical College of Wisconsin and in 2004 joined the LSU Health Sciences Center as Roland Coulson Professor and Head of the Department of Biochemistry & Molecular Biology. Dr. Haas has authored over 120 papers, reviews, and book chapters focused on cell regulation by ubiquitin and other Class 1 ubiquitin-like proteins, particularly ISG15. He has also served on numerous editorial boards, study sections, and scientific advisory panels in addition to visiting professorships at the University of Coimbra (Portugal), and the Institute of Biological Chemistry of the University of Urbino, Italy.

Clinical Interests

The roles of ubiquitin and ISG15 conjugation in cellular regulation.
 

Teaching Activities

The LSU School of Medicine and LSU Health Sciences Center are pleased to announce the establishment of the Arthur L. Haas Endowed Lectureship. An anonymous donor made a $100,000 gift to fund this lectureship in honor of Dr. Haas’ illustrious career in research, mentoring, and academic leadership and to support higher education within the School’s membership and the New Orleans public.read more...

Selected Publications

The active form of E6-associated protein (E6AP)/UBE3A ubiquitin ligase is an oligomer. Ronchi VP, Klein JM, Edwards DJ, Haas AL.J Biol Chem. 289, 1033-1048 (2014)

Identification of the major ubiquitin-binding domain of the Pseudomonas aeruginosa ExoU A2 phospholipase. Anderson DM, Feix JB, Monroe AL, Peterson FC, Volkman BF, Haas AL, Frank DW. J Biol Chem. 288, 26741-26752 (2013)

E6AP/UBE3A ubiquitin ligase harbors two E2-ubiquitin binding sites.  Ronchi, V.P., Klein, J.M.,  and  HAAS, A.L.  J. Biol. Chem. 288, 10349-10360 (2013).

TRIM ligases catalyze polyubiquitin chain formation through a cooperative allosteric mechanism.  Streich Jr., F.C., Ronchi, V.P., Connick, J.P.,  and  HAAS, A.L., J. Biol. Chem. 288, 8209-8221 (2013).

Secretory defect and cytotoxicity: The potential disease mechanisms for the retinitis pigmentosa (RP)-associated interphotoreceptor retinoid-binding protein. Li S., Yang Z., Hu J., Gordon W.C., Bazan N.G., HAAS, A.L., Bok D., Jin M., J. Biol. Chem. 288, 11395-406 (2013).

E2 binding surface on Uba3 β-grasp domain undergoes a conformational transition.  Elgin, S.E., Sökmen, N., Peterson, F.C., Volkman, B. F., Daǧ, Ç., and HAAS, A.L. Protein: Structure, Function, and Bioinformatics 80, 2482-2487 (2012).

ISG15 disrupts cytoskeletal architecture and promotes motility in human breast cancer cells.  Desai, S.D., Reed, R.E., Burks, J., Wood, L.M., Pullikuth, A.K., HAAS, A.L., Liu, L.F., Breslin, J.W., Meiners, S., and Sankar, S.  J. Exper. Biol. Med. 237, 38-49 (2012).

Ubiquitin and ubiquitin-modified proteins activate the Pseudomonas aeruginosa T3SS cytotoxin, ExoU.Anderson DM, Schmalzer KM, Sato H, Casey M, Terhune SS, Haas AL, Feix JB, Frank DW.Mol Microbiol.82, 1454-1467 (2011).

E1-E2 interactions in the ubiquitin and Nedd8 ligation pathways. Tokgöz Z, Siepmann TJ, Streich FC, Kumar B, Klein JM, Haas AL. J Biol Chem.287, 311-321 (2012).

Measuring rates of ubiquitin chain formation as a functional readout of ligase activity. Ronchi, VP and Haas AL. Meth. Mol. Biol., 832, 197-218 (2012).

A novel role for ATM in regulating proteasome-mediated protein degradation through suppression of the ISG15 conjugation pathway. Wood, LM, Sankar, S, Reed, RE, Haas AL, Liu, LF, McKinnon, P and Desai, SD. PloS ONE 6, e16422 (2011).

PARP-1 deficiency blocks IL-5 expression through calpain-dependent degradation of STAT-6 in a murine asthma model. Datta R, Naura AS, Zerfaoui M, Errami Y, Oumouna M, Kim H, Ju J, Ronchi VP, Haas AL, Boulares AH. PARP-1 deficiency blocks IL-5 expression through calpain-dependent degradation of STAT-6 in a murine asthma model. Allergy 66, 853-861 (2011).

Activation of ubiquitin and ubiquitin-like proteins. Streich, FC and Haas AL. Subcell.Biochem. 54, 1-16 (2010).

Ser120 of Ubc2/Rad6 regulates ubiquitin-dependent N-end rule targeting by E3a/Ubr1. Kumar, B, LeCompte, KG, Klein, JM and Haas AL. J. Biol. Chem. 285, 41300-41309 (2010).

Regulating the regulator: Rsp5 ubiquitinates the proteasome. Haas AL. Mol Cell. 5:623-624. (2010)

Structural biology: Transformative encounters.Schulman BA, Haas AL. Nature. 463:889-890. (2010)

Hdm2 is a ubiquitin ligase of Ku70-Akt promotes cell survival by inhibiting Hdm2-dependent Ku70 destabilization. Gama V, Gomez JA, Mayo LD, Jackson MW, Danielpour D, Song K, Haas AL, Laughlin MJ, Matsuyama. S. Cell Death Differ. 16:758-769. (2009)

Cho KI, Yi H, Desai R, Hand AR, Haas AL, Ferreira PA.RANBP2 is an allosteric activator of the conventional kinesin-1 motor protein, KIF5B, in a minimal cell-free system. EMBO Rep. 10:480-486. (2009)

Haas AL. Linear polyubiquitylation: the missing link in NF-kappaB signaling. Nat Cell Biol . 11:116-118.(2009)

Giannakopoulos NV, Arutyunova E, Lai C, Lenschow DJ, Haas AL, Virgin HW. ISG15 Arg151 and the ISG15-conjugating enzyme UbE1L are important for innate immune control of Sindbis virus. J Virol. 83:1602-1610. (2009)

Haas AL. Wilkinson KD. DeTEKting ubiquitination of APC/C substrates. Cell. 133:570-572. (2008)

Haas AL. Structural insights into early events in the conjugation of ubiquitin and ubiquitin-like proteins. Mol Cell. 27:174-175. (2007)

Sorensen CM, Rempel LA, Nelson SR, Francis BR, Perry DJ, Lewis RV, Haas AL, Hansen TR. The hinge region between two ubiquitin-like domains destabilizes recombinant ISG15 in solution.Biochemistry 46:772-780. (2007)

Melner MH, Haas AL, Klein JM, Brash AR, Boeglin WE, Nagdas SK, Winfrey VP, Olson GE. Demonstration of Ubiquitin Thiolester Formation of UBE2Q2 (UBCi), a Novel Ubiquitin Conjugating Enzyme with Implantation Site-Specific Expression.Biol. Reproduction 75:395-406. (2006)

Tokgoz Z, Bohnsack RN, Haas AL. Pleiotropic effects of ATP•Mg2+binding in the catalytic cycle of ubiquitin-activating enzyme. J Biol Chem. 281:14729-14737. (2006)

Haas, AL. ISG15-dependent regulation, in Protein Degradation, J. Mayer, A. Ciechanover, and R. Rechsteiner, eds., Wiley-VCH Verlag, Weinheim, Germany, 5:103-131. (2006)

Chen Y, Dai X, Haas AL, Wen R, Wang D. Proteasome-dependent down-regulation of activated Stat5A in the nucleus. Blood 108:566-574. (2006)

Desai SD, Haas AL, Wood LM, Tsai YC, Pestka S, Rubin EH, Saleem A, Nur-E-Kamal A, Liu LF. Elevated expression of ISG15 in tumor cells interferes with the ubiquitin/26S proteasome pathway. Cancer Res. 66:921-921. (2006)

Gama V, Yoshida T, Gomez JA, Basile DP, Mayo LD, Haas AL, Matsuyama S. Involvement of the ubiquitin pathway in decreasing Ku70 levels in response to drug-induced apoptosis.Exp Cell Res. 312:488-488. (2006)

Shang F, Deng G, Liu Q, Guo W, Haas AL, Crosas B, Finley D, Taylor A. Lys6-modified ubiquitin inhibits ubiquitin-dependent protein degradation. J Biol Chem. 280:20365-20365. (2005)

Narasimhan, J., Wang, M., Klein, J.M., Haas, AL*, and Kim, J.-J.P.*, Crystal structure of the interferon-induced ubiquitin-like protein ISG15. J. Biol. Chem. 280:27356-27365 (2005) (Cited by the Faculty of 1000; *corresponding authors) Reddy ST, Kumar SN, Haas AL, Dahms NM. Biochemical and functional properties of the full-length cation-dependent mannose 6-phosphate receptor expressed in Pichia pastoris. Biochem Biophys Res Commun. 309:643-651. (2003)

Kotamraju S, Tampo Y, Keszler A, Chitambar CR, Joseph J, Haas AL, Kalyanaraman B. N. Nitric oxide inhibits H2O2-induced transferrin receptor-dependent apoptosis in endothelial cells: Role of ubiquitin-proteasome pathway.Proc Natl Acad Sci U S A. 100:10653-8. (2003)

Bohnsack RN, Haas AL. Conservation in the mechanism of Nedd8 activation by the human AppBp1-Uba3 heterodimer. J Biol Chem.278(29):26823-26830. (2003)

Siepmann TJ, Bohnsack RN, Tokgoz Z, Baboshina OV, Haas AL. Protein interactions within the N-end rule ubiquitin ligation pathway.J Biol Chem. 278:9448-9457. (2003)

Badciong JC, Haas AL. MdmX is a RING finger ubiquitin ligase capable of synergistically enhancing Mdm2 ubiquitination.J Biol Chem. 277:49668-49675. (2002)

Wendelberger KJ, Burke R, Haas AL, Harenwattananon M, Simpson D. Identifying Opportunities for Vertical Integration of Biochemistry and Clinical Medicine. Adv Health Sci Educ Theory Pract. 3:157-164. (1998)

Balakirev MY, Jaquinod M, Haas AL, Chroboczek J. Deubiquitinating function of adenovirus proteinase. J Virol. 76:6323-6331. (2002)

Hamerman JA, Hayashi F, Schroeder LA, Gygi SP, Haas AL, Hampson L, CoughlinP, Aebersold R, Aderem A. Serpin 2a is induced in activated macrophages and conjugates to a ubiquitin homolog.J Immunol. 168:2415-2423. (2002)

Pru JK, Austin KJ, Haas AL, Hansen TR. Pregnancy and interferon-tau upregulate gene expression of members of the 1-8 family in the bovine uterus.Biol Reprod. 65:1471-1480. (2001)

Lawson TG, Sweep ME, Schlax PE, Bohnsack RN, Haas AL. Kinetic analysis of the conjugation of ubiquitin to picornavirus 3C proteases catalyzed by the mammalian ubiquitin-protein ligase E3alpha.J Biol Chem. 276:39629-39637. (2001)

Baboshina OV, Crinelli R, Siepmann TJ, Haas AL

N-end rule specificity within the ubiquitin/proteasome pathway is not an affinity effect. J Biol Chem. 276:39428-39437. (2001)

Macdonald JM, Haas AL. London RE. Novel mechanism of surface catalysis of protein a dduct formation. NMR studies of the acetylation of ubiquitin. J Biol Chem. 275:31908-31913. (2000)

Kurtzman AL, Gregori L, Haas AL, Schechter N. Ubiquitination and degradation of the zebrafish paired-like homeobox protein VSX-1.J Neurochem, 75:48-55. (2000)

Wilkinson KD, Laleli-Sahin E, Urbauer J, Larsen CN, Shih GH, Haas AL, Walsh ST, Wand AJ. The binding site for UCH-L3 on ubiquitin: mutagenesis and NMR studies on the complex between ubiquitin and UCH-L3.J Mol Biol. 291:1067-1077. (1999)

Potter JL, Narasimhan J, Mende-Mueller L, Haas AL. Precursor processing of pro-ISG15/UCRP, an interferon-beta-induced ubiquitin-like protein.J Biol Chem. 274:25061-25068. (1999)

Macdonald JM, LeBlanc DA, Haas AL, London RE. An NMR analysis of the reaction of ubiquitin with [acetyl-1-13C]aspirin. Biochem Pharmacol. 57:1233-1244. (1999)

Obin M, Mesco E, Gong X, Haas AL. Joseph J, Taylor A. Neurite outgrowth in PC12 cells. Distinguishing the roles of ubiquitylation andubiquitin-dependent proteolysis. J Biol Chem. 274:11789-11795. (1999)