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Cardiovascular Center of Excellence

NIH/NCRR

The Imaging and Histology Core
This Core will provide space, facilities, supervision and training for COBRE investigators who will use microscopy of live-cells and histological specimens (fixed and frozen) stained with various stains and immunohistochemical antibodies to answer specific aims outlined in their research projects.

The core provides skilled personnel to assist and/or perform highly specialized microscopic techniques including deconvolution, confocal microscopy, 3D reconstruction, co-localization, ratio imaging, fluorescent resonance energy transfer (FRET), and fluorescence recovery after photobleaching (FRAP) with both fixed and live cell samples. It also assists in the collection, processing and staining of histological samples. Standard staining includes H&E (hematoxylin and eosin). Special stains include Trichrome, Reticulin, PAS etc. as well as any relevant immunohistochemical antibody staining.

The core mentors junior and developing investigators regarding the potential application of microscopic techniques, histological analysis and immunohistochemistry to enhance their research projects and provide avenues towards developing translational research projects. In addition, the core guides investigators in image analysis methodologies to histological examination.

Dr. Andrew Catling will serve as the Core Director. He has extensive experience with the microscope instrumentation and cell/tissue histology services that this core provides. He will supervise training of all faculty on Core microscopes, will aid in experimental design and interpretation of data, and implement new techniques as they become available.

The state-of-art technology and experienced personnel provided by this Core will enhance the COBRE investigators progress towards acquisition of extramural funding and will support the overall mission of the Cardiovascular Center of Excellence.

The project described was supported by grant number P20RR018766 from the National Center of Research Resources (NCRR), a component of the National Institutes of Health (NIH) and its contents are solely the responsibility of the authors and do not necessarily report the official view of NCRR or NIH.

The project described was supported by grant number P20RR018766 from the National Center of Research Resources (NCRR), a component of the National Institutes of Health (NIH) and its contents are solely the responsibility of the authors and do not necessarily report the official view of NCRR or NIH.