BxMuscle_Nerve

PATHIST WEB

LOUISIANA STATE UNIVERSITY HEALTH SCIENCES CENTER / VETERANS AFFAIRS MEDICAL CENTER NEW ORLEANS

PATHOLOGY / HISTOTECHNOLOGY
PATOLOGIA / HISTOTECNOLOGIA DIAGNOSTIC PATHOLOGY
PATOLOGIA DIAGNOSTICA

INSTRUCTIONS ON HOW TO SUBMIT A MUSCLE BIOPSY TO THE LSUHSC
MUSCLE LABORATORY

DR. HERNAN CORREA
DEPARTMENT OF PATHOLOGY
1901 PERDIDO ST.- ROOM5D22
NEW ORLEANS,LA 70112-1393
TELEPHONE#(504)599-0281
FAX#(504)568-6037 MUSCLE HISTOCHEMISTRY
NERVE HISTOPATHOLOGY
HIGH RESOLUTION LIGHT MICROSCOPY
DIAGNOSTIC ELECTRON MICROSCOPY MODIFIED JUNE 20,2000

Local Navigational Box Caja de Navegación Local

Dx. Services Index

Indice Servicios de Dx

PLANNING THE MUSCLE BIOPSY

Designated person should call the Muscle Lab at LSUHSC (504)599-0281 in advance of scheduling the biopsy to make sure that someone will be there on that day to receive the muscle biopsy. Lab hours are 8a.m. to 4p.m., Monday through Friday.(Closed weekends and holidays.) Call the LSUHSC Pathology Department, Muscle Lab at (504)599-0281 or 568-6042 and ask to speak to Cheryl Vega or Teresa Flores. Please call again when you actually send the muscle biopsy to make sure someone in the lab will be waiting to receive the specimen.

PREPARATION OF THE MUSCLE BIOPSY SPECIMEN

The surgeon should remove a piece of muscle approximately 1.0x0.5x0.5cm. Ideally it should be cut so that the longitudinal orientation of the muscle fibers is easy to see. Place the muscle tissue in a clean, dry closed container, such as a screw-cap vial. DO NOT put the muscle in saline or wrap it in wet gauze, because saline washes out the glycogen. It is alright, however, to enclose moist gauze in the container, but not touching the muscle, to keep it from drying out. DO NOT tie the muscle with sutures, clamp it or stretch it on wood. The muscle tissue should then be sent to the hospital Pathology Lab, to be properly recorded and assigned an accession number. The specimen container is then packed in another container with crushed ice or ice cubes in it. This is to keep the specimen cool (refrigerator temperature) without freezing it. If you need to use ice packs instead of wet ice, put some paper towels between the specimen container and the ice packs so the muscle will not accidently freeze in transit. Enclose a completed, LSUHSC Muscle Biopsy Requisition Form with the specimen and send it to the LSUHSC Muscle Lab, Rm. 5D22, 1901 Perdido St. New Orleans, LA 70112 as soon as possible. Ideally a muscle biopsy specimen should reach the LSUHSC Muscle Lab within two hours of the surgery. Most hospitals pay a taxi or courier service to deliver the specimen. The delivery person must bring the package containing the muscle biopsy to Rm# 5D22, as the University Police will not accept packages. If it is necessary to send a specimen by overnight mail, package it as described above, using ice packs instead of wet ice and place container in a styrofoam box. DO NOT mail an overnight package on Friday.

THE FOLLOWING INFORMATION MUST BE ON THE REQUISITION FORM

Name & address of the hospital or clinic from which the specimen is sent
Name, date of birth and sex of the patient
Hospital identification number (Medical Records #)
Pathology specimen number
Which muscle was biopsied (or the location of the muscle biopsied)
Date of collection of specimen
Name and phone number of requesting physician
Clinical diagnosis or symptoms (History)
Name and address of physician to whom the report and slides should be sent if different from the Pathologist of the hospital that submitted the muscle biopsy.

ENCLOSE THE COMPLETED REQUISITION FORM (in a plastic bag) IN THE PACKAGE AND SUBMIT TO:
LOUISIANA STATE UNIVERSITY HEALTH SCIENCE CENTER
DEPARTMENT OF PATHOLOGY, MUSCLE LABORATORY
1901 PERDIDO ST. RM#5D22
NEW ORLEANS, LA 70112-1393
ATTN: CHERYL C. VEGA OR TERESA FLORES

Dr. Hernan Correa,a Board Certified Pathologist, will read the prepared battery of muscle enzyme stains and a report, photographs and a set of slides will be mailed to the Pathology Department of the hospital that submitted the muscle biopsy or the requesting physician within two weeks of receiving the muscle biopsy.

LSUHSC Muscle Laboratory will bill the Hospital that submitted the muscle biopsy a charge of $750.00. If Dystrophin Immunohistochemistry is required by Dr. H. Correa for proper diagnosis, there will be an additional charge of $50.00 per antibody.

INSTRUCTIONS ON HOW TO SUBMIT A NERVE BIOPSY TO THE LSUHSC NEUROMUSCULAR LABORATORY
Dx. Services Index / Indice Servicios de Dx

PLANNING THE NERVE BIOPSY

Designated person should call the Neuromuscular Lab at LSUHSC (504) 599-0281 in advance of scheduling the biopsy to make sure that someone will be there on that day to receive the biopsy. Lab hours are 7:30am-4:00pm Monday through Friday. (Closed weekends and holidays). Call the LSUHSC Pathology Dept., Neuromuscular Lab at (504)599-0281 or 568-6042 and ask to speak to Cheryl Vega or Teresa Flores.
Please call again when you actually send the nerve biopsy specimen to make sure someone in the lab will be waiting to receive the specimen.

PREPARATION OF THE NERVE BIOPSY SPECIMEN

Whole nerve biopsy rather than fascicular biopsy is preferred. Usually sural nerve is taken, but the superficial radial nerve at the wrist is also accessible. To minimize patient discomfort, the nerve should first be divided proximally. It then may be carefully dissected free and the distal end cut without futher pain. After removal, the nerve should be laid straight and stretched on a piece of tongue blade by carefully pulling on the ends of the nerve with forceps and allowing it to adhere for a few seconds. Pinning is not necessary. The piece of wood should be placed so that the nerve is on the down side and submerged in the fixative. Do not delay fixation or allow the nerve to dry out. Either paraformaldehyde EM fixative or 10% neutral buffered formalin may be used to fix the nerve. The nerve specimen should then be sent to the hospital Pathology Lab, to be properly recorded and assigned an accession number. The specimen may be sent to LSUHSC Lab the same day or allowed to fix over-night, removed from the wood, and then sent. The latter is preferable because the nerve is more likely to remain submerged in the fixative while in transit if it is not on the wood. Most hospitals pay a taxi driver to deliver the specimen

THE FOLLOWING INFORMATION MUST BE ON THE REQUISITION

Name and address of the hospital or clinic from which the specimen is being sent.
Name, age, birthday and sex of the patient.
Hospital or Clinic identification number (Medical Records #).
Pathology specimen number.
Which nerve was biopsied (or location of the nerve biopsied).
Date of collection of the specimen.
Name and phone number of the referring physician.
Whom the report and slides should be sent to.

Our Neuromuscular Lab generally mails the report and slides to the Pathology of the hospital that submitted the nerve biopsy.

Enclose the Requisition Form and specimen in separate plastic bags and submit to:

Louisiana State University Health Science Center
Neuromuscular Lab, Rm. 5D22, Cheryl Vega 1901 Perdido Street
New Orleans, LA. 70112-1393
Telephone:(504)599-0281
The delivery person must bring the package containing the nerve biopsy to the 5th floor,
Room 5D22 as the University Police will not accept packages.

Dr. Hernan Correa, who is a board certified Pathologist, will read the prepared specimen and a report, photographs and a set of slides will be mailed to the Pathology Department that submitted the nerve biopsy or the designated physician.

LSUHSC Neuromuscular Lab will bill the hospital that submitted the nerve biopsy charge of $750.00.

PATHIST WEB		LOUISIANA STATE UNIVERSITY HEALTH SCIENCES CENTER / VETERANS AFFAIRS MEDICAL CENTER NEW ORLEANS
PATHOLOGY / HISTOTECHNOLOGY PATOLOGIA / HISTOTECNOLOGIA		DIAGNOSTIC PATHOLOGY PATOLOGIA DIAGNOSTICA
INSTRUCTIONS ON HOW TO SUBMIT A MUSCLE BIOPSY TO THE LSUHSC MUSCLE LABORATORY
DR. HERNAN CORREA DEPARTMENT OF PATHOLOGY 1901 PERDIDO ST.- ROOM5D22 NEW ORLEANS,LA 70112-1393 TELEPHONE#(504)599-0281 FAX#(504)568-6037		MUSCLE HISTOCHEMISTRY NERVE HISTOPATHOLOGY HIGH RESOLUTION LIGHT MICROSCOPY DIAGNOSTIC ELECTRON MICROSCOPY MODIFIED JUNE 20,2000
Local Navigational Box		Caja de Navegación Local
*Dx. Services Index*		*Indice Servicios de Dx*
PLANNING THE MUSCLE BIOPSY Designated person should call the Muscle Lab at LSUHSC (504)599-0281 in advance of scheduling the biopsy to make sure that someone will be there on that day to receive the muscle biopsy. Lab hours are 8a.m. to 4p.m., Monday through Friday.(Closed weekends and holidays.) Call the LSUHSC Pathology Department, Muscle Lab at (504)599-0281 or 568-6042 and ask to speak to Cheryl Vega or Teresa Flores. Please call again when you actually send the muscle biopsy to make sure someone in the lab will be waiting to receive the specimen.
PREPARATION OF THE MUSCLE BIOPSY SPECIMEN The surgeon should remove a piece of muscle approximately 1.0x0.5x0.5cm. Ideally it should be cut so that the longitudinal orientation of the muscle fibers is easy to see. Place the muscle tissue in a clean, dry closed container, such as a screw-cap vial. DO NOT put the muscle in saline or wrap it in wet gauze, because saline washes out the glycogen. It is alright, however, to enclose moist gauze in the container, but not touching the muscle, to keep it from drying out. DO NOT tie the muscle with sutures, clamp it or stretch it on wood. The muscle tissue should then be sent to the hospital Pathology Lab, to be properly recorded and assigned an accession number. The specimen container is then packed in another container with crushed ice or ice cubes in it. This is to keep the specimen cool (refrigerator temperature) without freezing it. If you need to use ice packs instead of wet ice, put some paper towels between the specimen container and the ice packs so the muscle will not accidently freeze in transit. Enclose a completed, LSUHSC Muscle Biopsy Requisition Form with the specimen and send it to the LSUHSC Muscle Lab, Rm. 5D22, 1901 Perdido St. New Orleans, LA 70112 as soon as possible. Ideally a muscle biopsy specimen should reach the LSUHSC Muscle Lab within two hours of the surgery. Most hospitals pay a taxi or courier service to deliver the specimen. The delivery person must bring the package containing the muscle biopsy to Rm# 5D22, as the University Police will not accept packages. If it is necessary to send a specimen by overnight mail, package it as described above, using ice packs instead of wet ice and place container in a styrofoam box. DO NOT mail an overnight package on Friday.
THE FOLLOWING INFORMATION MUST BE ON THE REQUISITION FORM Name & address of the hospital or clinic from which the specimen is sent Name, date of birth and sex of the patient Hospital identification number (Medical Records #) Pathology specimen number Which muscle was biopsied (or the location of the muscle biopsied) Date of collection of specimen Name and phone number of requesting physician Clinical diagnosis or symptoms (History) Name and address of physician to whom the report and slides should be sent if different from the Pathologist of the hospital that submitted the muscle biopsy. ENCLOSE THE COMPLETED REQUISITION FORM (in a plastic bag) IN THE PACKAGE AND SUBMIT TO: LOUISIANA STATE UNIVERSITY HEALTH SCIENCE CENTER DEPARTMENT OF PATHOLOGY, MUSCLE LABORATORY 1901 PERDIDO ST. RM#5D22 NEW ORLEANS, LA 70112-1393 ATTN: CHERYL C. VEGA OR TERESA FLORES Dr. Hernan Correa,a Board Certified Pathologist, will read the prepared battery of muscle enzyme stains and a report, photographs and a set of slides will be mailed to the Pathology Department of the hospital that submitted the muscle biopsy or the requesting physician within two weeks of receiving the muscle biopsy. LSUHSC Muscle Laboratory will bill the Hospital that submitted the muscle biopsy a charge of $750.00. If Dystrophin Immunohistochemistry is required by Dr. H. Correa for proper diagnosis, there will be an additional charge of $50.00 per antibody.
INSTRUCTIONS ON HOW TO SUBMIT A NERVE BIOPSY TO THE LSUHSC NEUROMUSCULAR LABORATORY *Dx. Services Index / Indice Servicios de Dx*
PLANNING THE NERVE BIOPSY Designated person should call the Neuromuscular Lab at LSUHSC (504) 599-0281 in advance of scheduling the biopsy to make sure that someone will be there on that day to receive the biopsy. Lab hours are 7:30am-4:00pm Monday through Friday. (Closed weekends and holidays). Call the LSUHSC Pathology Dept., Neuromuscular Lab at (504)599-0281 or 568-6042 and ask to speak to Cheryl Vega or Teresa Flores. Please call again when you actually send the nerve biopsy specimen to make sure someone in the lab will be waiting to receive the specimen.
PREPARATION OF THE NERVE BIOPSY SPECIMEN Whole nerve biopsy rather than fascicular biopsy is preferred. Usually sural nerve is taken, but the superficial radial nerve at the wrist is also accessible. To minimize patient discomfort, the nerve should first be divided proximally. It then may be carefully dissected free and the distal end cut without futher pain. After removal, the nerve should be laid straight and stretched on a piece of tongue blade by carefully pulling on the ends of the nerve with forceps and allowing it to adhere for a few seconds. Pinning is not necessary. The piece of wood should be placed so that the nerve is on the down side and submerged in the fixative. Do not delay fixation or allow the nerve to dry out. Either paraformaldehyde EM fixative or 10% neutral buffered formalin may be used to fix the nerve. The nerve specimen should then be sent to the hospital Pathology Lab, to be properly recorded and assigned an accession number. The specimen may be sent to LSUHSC Lab the same day or allowed to fix over-night, removed from the wood, and then sent. The latter is preferable because the nerve is more likely to remain submerged in the fixative while in transit if it is not on the wood. Most hospitals pay a taxi driver to deliver the specimen
THE FOLLOWING INFORMATION MUST BE ON THE REQUISITION Name and address of the hospital or clinic from which the specimen is being sent. Name, age, birthday and sex of the patient. Hospital or Clinic identification number (Medical Records #). Pathology specimen number. Which nerve was biopsied (or location of the nerve biopsied). Date of collection of the specimen. Name and phone number of the referring physician. Whom the report and slides should be sent to. Our Neuromuscular Lab generally mails the report and slides to the Pathology of the hospital that submitted the nerve biopsy. Enclose the Requisition Form and specimen in separate plastic bags and submit to: Louisiana State University Health Science Center Neuromuscular Lab, Rm. 5D22, Cheryl Vega 1901 Perdido Street New Orleans, LA. 70112-1393 Telephone:(504)599-0281 The delivery person must bring the package containing the nerve biopsy to the 5th floor, Room 5D22 as the University Police will not accept packages. Dr. Hernan Correa, who is a board certified Pathologist, will read the prepared specimen and a report, photographs and a set of slides will be mailed to the Pathology Department that submitted the nerve biopsy or the designated physician. LSUHSC Neuromuscular Lab will bill the hospital that submitted the nerve biopsy charge of $750.00.

PATHIST WEB

LOUISIANA STATE UNIVERSITY HEALTH SCIENCES CENTER / VETERANS AFFAIRS MEDICAL CENTER NEW ORLEANS