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Special Stains

SPECIAL STAINS PERFORMED AT THE SURGICAL PATHOLOGY LABORATORY
Dr. Carmen Espinoza

TITLE: Special stains performed at MCLNO laboratory
EFFECTIVE DATE: March 2, 2004
APPROVED BY: Carmen G. Espinoza, SECTION DIRECTOR

Department of Pathology
Louisiana State University Medical Center. 1901 Perdido Street. New Orleans. LA (504) 568-6032. Fax: (504)568-6037

SPECIAL STAINS

Volume II of our Laboratory Manuals is dedicated to special stains offered in the Surgical Pathology Laboratory. The manual is available in the Histology Laboratory area. Common requested stains are done in a Ventana automatic stainer.

TURNAROUND TIME FOR SPECIAL STAINS

Special Stains requested by 9 A.M. are performed the same day. Special Stains requested after 9 A.M. are performed the following day.

SPECIAL STAINS PERFORMED IN THE LABORATORY:

ELEMENTS STAINED

NAME OF STAIN

Microorganisms

Acid-Fast Organism Fite stain
Bacteria, blood elements May - Grunwald Giemsa Stain
Bacteria Gram stain
Bacteria/Protozoa Lillie's Azure A Eosin B Stain
Helicobacter pylori Toluidine Method
Helicobacter pylori Alcian yellow, automated
Fungi Gomori's methanamine silver method, both manual and automated
Fungi PAS green, both manual and automated
Fungi/Cyptococcus Mucicarmine
Spirochetes Steiner, automated.
Spirochetes Warthin - Starry microwave method

Connective tissue and lipids

Collagen Gomori's One-Step Trichrome Stain manual, Trichrome automated
Elastic fibers Verhoeff - Van Gieson stain
Reticulin Wilder's method, both manual and automated
Fat Oil red O stain for frozen section

Carbohydrates

Glycogen/neutral mucin Lillie PAS stain manual, PAS automated
Glycogen digestion Amylase digestion procedure, both manual and automated.
Mucopolysaccharid-acid Modified Mowry's Colloidal iron stain
Mucopolysaccharid-acid Alcian Blue pH 2.5 or pH 1.0
Acid and neutral mucopolysaccharides Alcian blue 2.5 and PAS
Sialomucin Mayer's mucicarmine stain

Amyloid

Amyloid Alkaline Congo Red stain
Amyloid Lieb's crystal violet method

Minerals, pigments and miscellaneous

Calcium Kossa's method
Iron Gomori's method
Melanin Fontana Masson Silver stain
Melanin removal Permanganate method
Charcott Leyden crystals Luna stain
Muscle striations Mallory's PTAH stain