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PAS PAS with amylase digestion Colloidal iron Alcian blue

Lillie PAS stain
CONTROL TISSUE: Exocervix, uterus
MAIN DISTRIBUTION: Glycogen in squamous epithelium
TYPE OF STAINING: Magenta
APPLICATION: Detection of glycogen and neutral mucins, stains basement membranes, fungal walls.
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PAS after Amylase digestion for glycogen

CONTROL TISSUE: Exocervix, uterus , same control for PAS
TYPE OF STAINING: Notice that all glycogen is gone. Basement membrane and neutral mucin staining persists.
APPLICATION: To demonstrate that the PAS positive staining substance is glycogen.

 
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Modified Mowry's colloidal iron stain
CONTROL TISSUE: Colonic mucosa
MAIN DISTRIBUTION: acid mucopolysaccharides in glands and connective.
TYPE OF STAINING: Deep (Prussian) blue
APPLICATION: connective tissue mucinosis.
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Alcian blue
CONTROL TISSUE: colonic mucosa
MAIN DISTRIBUTION: mucin
TYPE OF STAINING: blue cytoplasm
APPLICATION: Acid mucopolysaccharides detection.
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