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Calcium Iron Melanin Muscle striations

Kossa's method for calcium

CONTROL TISSUE: coronary artery with calcified atheromatous plaque
MAIN DISTRIBUTION: calcified areas
TYPE OF STAINING: dark granular
APPLICATION: Identification of calcium.
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Gomori stain for iron

CONTROL TISSUE: Liver with hemochromatosis
MAIN DISTRIBUTION: Macrophages and parenchymal cells
TYPE OF STAINING:The iron pigment which is brown on H&E turns blue.
APPLICATION: Identification of iron..

 
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Fontana masson silve stain for melanin

CONTROL TISSUE: skin
MAIN DISTRIBUTION: Melanin pigment
TYPE OF STAINING: Black color.
APPLICATION: Identification of melanin pigment.

Note: Melanin, brown or black on H&E will turn green with methylene blue or toluidine blue.
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Mallory's PTAH stain
CONTROL TISSUE: Skeletal muscle
MAIN DISTRIBUTION: muscle cells
TYPE OF STAINING: Purple
APPLICATION: Identification of skeletal muscle striations in tumor with rhabdomyoblasts.
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