Hamid Boulares, PhD

Research

We focus on the use of cell culture systems and animal models to study the intricate relation between poly(ADP) ribose polymerase–1 (PARP-1) and oxidative stress in asthma, atherosclerosis, and colon cancer.

Publications

Boulares, H and Ren, T (2004). Mechanism of acetaminophen-induced apoptosis: Roles of caspase-3, DNA fragmentation factor, and the Ca2+ and Mg2+ endonuclease DNAS1L3. Pharmacol. Toxicol. 94: 19-29.

Boulares, A.H., Zoltoski, AJ., Sherif, Z., Jolly, p., Massaro, D., and Smulson, ME. Gene knockout or pharmacological inhibition of poly(ADP-ribose) polymerase-1 prevents lung inflammation in a murine model of asthma (2003). Am. J. Respir. Cell Mol. Biol. 28: 322-329.

Boulares, A.H., Zoltoski, AJ., Sherif, Z., Yakovlev, AG., and Smulson, ME. DNAS1L3, a PARP-1-regulated endonuclease, is required for internucleosomal DNA fragmentation and increases sensitivity of transfected osteosarcoma cells in response to etoposide (2002). Cancer Res. 62:4439-44.

Boulares, A.H., Zoltoski, A., Yakovlev, A, Contreras, FJ., and Smulson, ME. (2002) Regulation of DNAS1L3 endonuclease activity by poly(ADP-ribosyl)ation during etoposide-induced apoptosis: role of PARP-1 cleavage in endonuclease activation. J. Biol. Chem. 277: 372-378.

Boulares, A.H., Zoltoski, A. J., Yakovlev, A.G., Xu, M., and Smulson M.E. (2001). Roles of DNA fragmentation factor and poly(ADP-ribose) polymerase in an amplification phase of tumor necrosis factor–induced apoptosis. J. Biol. Chem. 276: 38185-38192.

Boulares, AH., Yakovlev, A., Ivanova, V., Stoica, B., Iyer, S., and Smulson M. (1999). Role of PARP cleavage in apoptosis: caspase 3 resistant PARP mutant increases rates of apoptosis in transfected cells. J. Biol. Chem. 274: 22932-22940.

Giardina, C., Boulares, H., Inan, S. (1999). NSAIDs and butyrate sensitize a human colorectal cancer cell line to TNF-? and Fas ligation: the role of reactive oxygen species. Biochim. Biophys. Acta. 1448: 425-438.

Boulares, AH., Giardina, C., Navarro, C., Khairallah, E. and Cohen S. (1999). Modulation of serum growth factors signal transduction in Hepa 1-6 cells by acetaminophen: an inhibition of c-myc expression, NF-?B activation, and Raf-1 kinase activity. Toxicol. Sci. 48: 264-274.

Boulares, AH., Giardina, C., Inan, M., Khairallah, E. and Cohen S. (2000) Acetaminophen inhibits H2O2-induced NF-?B activation by interfering with the oxidant signal in murine Hepa 1-6 cells. Toxicol Sci. 55:370-5.

Yakovlev, A., Wang, G., Stoica, B., Boulares, A. H., Spoonde, A., and Smulson, M. (2000) Regulation of Ca2+/Mg2+-dependent endonuclease activity in apoptosis: Inhibition of DNAS1L3 by Poly(ADP-ribose)polymerase. J Biol Chem. 275: 21302-8.

Stoica, B., Boulares, AH., Rosenthal DS, Iyer S, Hamilton, G., and Smulson M. E. (2001). Mechanisms of JP-8 Jet Fuel Toxicity. I. Induction of Apoptosis in Rat Lung Epithelial Cells. Toxicol. Appl Pharmacol. 171:94-106.

Yakovlev, A.G., Di, X., Movsesyan, V., Mullins, P.G., Wang, G., Boulares, H., Zhang, J., Xu, M., and Faden, A. I. (2001) Presence of DNA fragmentation and lack of neuroprotective effect in DFF45 knockout mice subjected to traumatic brain injury. Mol. Med. 7: 205-216.

Boulares, A.H., Zoltoski, A.J., Stoica, B., and Smulson, M. E. (2002) Acetaminophen induces a caspase-dependent and Bcl-xL sensitive apoptosis in human hepatoma cells and lymphocytes. Pharmacol. Toxicol. 90: 38-50.

Boulares, A.H., Contreras, F., Espinoza, L., and Smulson, M. Roles of glutathione and oxidative stress in JP-8 jet fuel-induced apoptosis in rat lung epithelial cells. (2002). Toxicol. Appl. Pharmacol. 180: 92-99.