Cover figures from the lab

Breeding strategy to generate triple transgenic SARA mice overexpressing human angiotensionogen, renin and angiotensin converting enzyme type 2 in the central nervous system; bottom left: immunostaining for the Mas receptor in the paraventricular nucleus of a syn-hACE2 transgenic mouse overexpressing human ACE2 specifically in neurons; bottom right: spontaneous baroreflex sensitivity in wildtype (left), chronically hypertensive R+A+ double transgenic mice (middle) and triple transgenic SARA mice (right).

The colocalization of the human Angiotensin Converting Enzyme type 2 (ACE2; red) and mouse Microtubule-Associated Protein-2 (MAP2; green), a marker for neurons and 4”,6-diamidino-2-phenylindole (DAPI; blue), a nucleic acid marker, in the syn-hACE2 transgenic mouse rostral ventrolateral medulla (RVLM) detected by immunofluorescence. Overlapping fluorescence (yellow) indicates that the human ACE2 transgene expression is specifically targeted to neurons. Magnification: 60X.

Human angiotensin-converting enzyme 2 (hACE2) expression in neurons of rostral ventrolateral medulla (RVLM) in triple transgenic model (SARA) mice. A, Immunostaining for hACE2 (red), microtubule associated protein-2 (MAP-2; neuronal marker, green), and 4′,6-diamidino-2-phenylindole (DAPI; nucleic acid marker, blue) in the RVLM of a SARA mouse (magnification ×10). B, Increased magnification (×40) shows the presence of hACE2 in cells of the RVLM. C, MAP-2 immunofluorescence shows the distribution of neurons in the RVLM (×40). D, Overlapping fluorescence (yellow) confirms the presence of hACE2 on neurons in the RVLM (×40).

 

Lazartigues Laboratory
2015 9:58:36 AM

From Gene to Function: the Role of the Renin-Angiotensin System in the Regulation of Cardiovascular Function .

 

News from the Lab: 

  • Ms. Alynne Carvalho-Galvão's manuscript entitled Central administration of TRV027 improves baroreflex sensitivity and vascular reactivity in spontaneously hypertensive rats was accepted for publication in Clinical Sciences. (6/2018) 
  • Dr. Jiaxi Xu's  manuscript entitled Excessive Glutamate Stimulation Impairs ACE2 Activity Through ADAM17-Mediated Shedding in Cultured Cortical Neurons was accepted for publication in Cellular and Molecular Neurobiology. (5/2018)
  • Dr. Lazartigues and his colleague Dr. Catalin Filipeanu (Howard Univ.) received a 4-year Merit Award (I01 BX004294) from the U.S. Department of Veterans Affairs. (5/2018)
  • Dr. Lazartigues and his colleagues Drs. Yue and Gardner (Physiology), were awarded a multiple PI R01 (1R01HL135635) entitled "Chronic nicotine inhalation increases susceptibility to cardiovascular and pulmonary diseases through inhibition of local compensatory mechanisms" (12/2016). 
  • Ms. Harshita Chodavarapu and Dr. Kavaljit Chhabra's manuscript entitled High-fat diet-induced glucose dysregulation is independent of changes in islet ACE2 in mice was accepted for publication in the American Journal of Physiology, Regulatory, Integrative and Comparative Physiology. (10/2016)
  • Dr. Jiaxi Xu and Ms. Snigdha Mukerjee's manuscript entitled A disintegrin and metalloprotease 17 in the cardiovascular and central nervous systems was accepted for publication in Frontiers in Physiology. (10/2016).
  • Dr. Srinivas Sriramula made an oral presentation entitled œKnockdown of Bradykinin B1 Receptor Reduces Neuro-inflammation and Prevents the Development of Salt-Sensitive Hypertension (Experimental Biology meeting, San Diego, CA, April 2-6th, 2016).
  • Dr. Jiaxi Xu made an oral presentation entitled Neuronal activation of ADAM17 by AT1A receptors contributes to neurogenic hypertension. Dr. Xu received the Postdoctoral and Early Career Research Recognition Award from the American Physiological Society Neural Control and Autonomic Regulation section (Experimental Biology meeting, San Diego, CA, April 2-6th, 2016).
  • Ms. Snigdha Mukerjee made an oral presentation entitled ACE2 Deletion is Associated with Increased ADAM17 and Reduced Inhibitory Currents to Pre-sympathetic Hypothalamic Neurons. Ms. Mukerjee received the Predoctoral Research Distinction Award from the American Physiological Society Water & Electrolyte section (Experimental Biology meeting, San Diego, CA, April 2-6th, 2016).
  • Dr. Jiaxi Xu made an oral presentation entitled ADAM17 in Neurogenic Hypertension: Function and Regulation. Dr. Xu received the Gordon Research Conference Poster Award (Angiotensin Gordon Research Conference, Il Ciocco, Italy, February 21-26th, 2016).
  • Ms. Snigdha Mukerjee made an oral presentation entitled ACE2 modulates the excitability of pre-sympathetic neurons in the paraventricular nucleus (X International Symposium on Vasoactive Peptides, Lagoa dos Ingleses, Minas Gerais, Brazil, October 29-31st 2015:).
  • Dr. Jiaxi Xu was awarded a 2-year Post-doctoral fellowship from the American Heart Association Greater SouthEast Affiliate to study the role of ADAM17 and AT1 receptors in neurogenic hypertension (July 2015).
  • Dr. Srinivas Sriramula was awarded a 3-year Scientist Development Grant from the American Heart Association Greater SouthEast Affiliate to study the role of bradykinin receptors in neurogenic hypertension (July 2015).

 

 

 

 

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