Postdoctoral Training – 2002-2004, Departments of Medical Physiology and Surgery, Texas A&M University Health Science Center/Scott and White Memorial Hospital

Postdoctoral Training – 2004-2007, Department of Surgery, UC Davis School of Medicine

Bio

My research focuses on the impact of inflammation on microvascular permeability and lymphatic clearance of excess fluids from tissues. We use a multilevel approach combining in vivo, isolated tissue, and cell culture models in my laboratory. Our ongoing goal is to characterize new molecular signaling pathways that could serve as potential targets to reduce or resolve excessive microvascular leakage and edema.

Research Interests

Signal Transduction in Enhanced Microvascular Permeability

Mechanisms of Lymph Formation and Propulsion

Impact of Alcohol Intoxication on the Gut Microcirculation and Lymphatics

Calcium-Dependent/Sensitizing Mechanisms Underlying the Lymphatic Contractile Cycle

Endothelial Cell Biology

Teaching Activities

Areas of Expertise: Vascular Physiology, Gastrointestinal Physiology, Cell Physiology

Course Directorship: PHYSIO 281: Special Topics: Journal Club

Course Co-Directorship: Dental Physiology 1115

Selected Publications

Doggett TM, Breslin JW. Study of actin dynamics in endothelial cells expressing GFP-actin. J Vis. Exp., 57: e3187, DOI: 10.3791/3187, 2011. Link: http://www.jove.com/video/3187/study-of-the-actin-cytoskeleton-in-live-endothelial-cells-expressing-gfp-actin

Souza-Smith FM, Kurtz, KM, Breslin JW. Measurement of cytosolic Ca²⁺ in isolated contractile lymphatics. J Vis. Exp. 58: e3438, DOI: 10.3791/3438, 2011.
Link: http://www.jove.com/video/3438/measurement-of-cytosolic-ca2-in-isolated-contractile-lymphatics

Breslin JW. ROCK and cAMP promote lymphatic endothelial cell barrier integrity and modulate histamine and thrombin-induced barrier dysfunction. Lymphat. Res. Biol. 9:3-11, 2011. PMID: 21417762. Link: http://dx.doi.org/10.1089/lrb.2010.0016

Souza-Smith FM, Kurtz KM, Molina PE, Breslin JW. Adaptation of intrinsic mesenteric lymphatic function following acute alcohol intoxication. Microcirculation 17: 514-524, 2010. PMID: 21040117.
Link: http://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21040117/?tool=pubmed

Duran, W.N., Breslin, J.W., Sanchez, F.A., The NO cascade, eNOS location, and microvascular permeability., Cardiovasc Res. (in Press), 2010. PMID: 20462865.
Link: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2895543/?tool=pubmed

Breslin, J.W., Kurtz, K.M., Lymphatic endothelial cells adapt their barrier function in response to changes in shear stress., Lymphat Res Biol 7:229-237, 2009. PMID: 20143922.
Link: http://www.ncbi.nlm.nih.gov/pubmed/20143922

Breslin JW, Sun H, Xu W, Rodarte C, Moy AB, Wu MH, Yuan SY. Involvement of ROCK-mediated endothelial tension development in neutrophil-stimulated microvascular leakage. Am. J. Physiol. Heart Circ. Physiol. 290: H741-H750, 2006. PMID: 16172166 PMCID: PMC2802275

Additional Info

Curriculum Vitae

Pubmed Link

Grant Support:

NIH 1R01HL098215-01A1 “Regulatory Mechanisms for Resolution of Inflammatory Microvascular Leakage”

NIH/NIAAA 1R21AA020049-01A1 “Impact of Alcohol Intoxication on Hemorrhagic Shock-Induced Microvascular Dysfunction”

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