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Proteomics Core

Instrumentation

The Core Facility is well equipped with instrumentations in 2 dimensional gel electrophoresis, liquid chromatography and mass spectrometry, for sample separation, analysis, and ultimate detection.

The major pieces of equipment serving the facility are:

2D Gel Electrophoresis

• 2D DIGE:

-Amersham IPGphor and Ettan DALT 2D gel electrophoresis
-Typhoon imaging system (for visualization of fluorescence dyes)
-DeCyder software program for image analysis and spot processing
-Ettan liquid sample handling robot workstation

• Traditional 2D gel electrophoresis

-BioRad 2D gel electrophoresis system
-BioRad GS-800 densitometer (for visible stains and films)
-ProteomeWorks Spot Cutter with PDQuest software for gel imaging analysis and spot picking

Mass Spectrometer

• Thermo-Fisher LTQ XL Linear Ion Trap mass spectrometer coupling with Eskigent nanoLC liquid chromatographer. (Current Status: working)
• AB Sciex (formerly part of Applied Biosystems) 4700 Proteomics Analyzer Matrix Assisted Laser Desorption Ionization Time-of-Flight mass spectrometer. (Current Status: not working)
• AB Sciex Voyager DE Matrix-Assisted Laser Desorption Ionization  Time-of-Flight mass spectrometer. (Current Status: not working)

• Dionex Ultimate 3000 Multidimensional Liquid Chromatographer with UV/Vis detector
• Agilent 1000 Liquid Chromatographer with UV/Vis detector (status: not working)

Additional auxiliary equipment:

• UV/Vis SmartSpec spectrophotometer
  
• Eppendorf Vacufuge (vacuum centrifuge)
  
• Barnstead Nanopure Diamond water purification system