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Proteomics Core
Services and Costs
Service
Currently, the facility starts to offer limited service. The followings
are the draft of services and charge. We anticipate implementing the
full services in September 2004. In the future, we hope to expand
the facility service through grant funding with the collaboration
of faculty in LSU. Please contact to Dr. Chou for future information
about the currently available service, sample preparation and scheduling,
etc.
Investor Cost
The services and costs for investors
in the Proteomics Core Facility will be described as followings:
| Sample Processing |
Charging Unit |
Cost |
| Automated in-gel trypsin digest (gel slice subjected to destaining and
in gel digestion) |
per sample or per spot, whichever is applicable |
First 20 samples-$14.44 per spot, $6.98 for additional spot |
| ZipTip sample cleanup |
per sample |
$14.44 |
MALDI (4700)
| Sample Processing |
Charging Unit |
Cost |
| Simple mass spectral analysis, molecular weight information (linear
or reflector mode, peak list and spectrum, not for protein identification)
|
per sample |
First 20 samples-$40.03/sample, $20.08 for additional samples |
| Advanced automated reflector spectral analysis with database search
(MS, MS/MS of up to 5 peptide fragments per sample and database search/protein
identification) |
per sample |
First 20 samples-$50 per sample, $25 for additional sample |
| Additional MS/MS analyses after 5 fragments |
per peptide fragment ion |
$4.96/fragment |
| de novo/ProBlast search of MS/MS of a peptide (manual) |
per sample |
$63.62 |
| Experiments performed on the archiveable single-use sample plate |
additional charge on per plate |
$47.64 |
| Additional data analyses (spectral analysis, database search,
etc.) |
per hour |
$72.79 |
2-D gel electrophoresis
| Sample Processing |
Charging Unit |
Cost |
| Experiment performed by the Core Facility personnel on a Bio-Rad system
(IEF, PAGE, staining and scan; best with silver and coomassie staining
methods; fluorescence stain/CyDye is extra) |
per gel |
11-cm gel $90.36/gel, 18-cm gel $142.28/gel, Inquire for other sizes |
| Training fee for new users (PDQuest scanning and imaging analysis).
Free for authorized users. |
per hour |
$45.77/hr, no charge after training. |
| Experiment performed by the Core Facility personnel on an Amersham Ettan
system (IEF, PAGE, staining and scan; best for fluorescence visualization
methods; fluorescence stain/CyDye is extra) |
per gel |
Inquiry |
| Gel scanning and spot picking (with Core personnel) |
per hour |
$45.77/hr |
| Coomassie staining or silver staining |
per gel |
Free |
| Cy-Dye minimum labeling (Cy2, Cy3, Cy5) |
per gel |
Inquiry |
| Sypro Ruby staining |
per gel |
$25.08 for 11-cm gel, $48.13 for 24-cm gel |
| User fee for authorized users (BioRad system 2-D gel electrophoresis
apparatus, reagents) |
per use |
IEF $35, PAGE $10/hr ($60 maximum/use) |
Please contact Core Facility for special services
Non-Investor Cost
The services and costs for non-investors
in the Proteomics Core Facility will be described as followings:
| Sample Processing |
Charging Unit |
Cost |
| Automated in-gel trypsin digest (gel slice subjected to destaining and
in gel digestion) |
per sample or per spot, whichever is applicable |
First 20 samples-$22.88 per spot, $14.46 for additional spot |
| ZipTip sample cleanup |
per sample |
$20.18 |
MALDI (4700)
| Sample Processing |
Charging Unit |
Cost |
| Simple mass spectral analysis, molecular weight information (linear
or reflector mode, peak list and spectrum, not for protein identification)
|
per sample |
First 20 samples-$51.90/sample, $29.92 for additional samples |
| Advanced automated reflector spectral analysis with database search
(MS, MS/MS of up to 5 peptide fragments per sample and database search/protein
identification) |
per sample |
First 20 samples-$54.96 per sample, $35.06 for additional sample |
| Additional MS/MS analyses after 5 fragments |
per peptide fragment ion |
$8.05/fragment |
| de novo/ProBlast search of MS/MS of a peptide (manual) |
per sample |
$80.19 |
| Experiments performed on the archiveable single-use sample plate |
additional charge on per plate |
$47.39 |
| Additional data analyses (spectral analysis, database search,
etc.) |
per hour |
$70.85 |
2-D gel electrophoresis
| Sample Processing |
Charging Unit |
Cost |
| Experiment performed by the Core Facility personnel on a Bio-Rad system
(IEF, PAGE, staining and scan; best with silver and coomassie staining
methods; fluorescence stain/CyDye is extra) |
per gel |
11-cm gel $107.49/gel, 18-cm gel $160.00/gel, Inquire for other sizes |
| Training fee for new users (PDQuest scanning and imaging analysis).
Free for authorized users. |
per hour |
$60.35/hr |
| Experiment performed by the Core Facility personnel on an Amersham Ettan
system (IEF, PAGE, staining and scan; best for fluorescence visualization
methods; fluorescence stain/CyDye is extra) |
per gel |
Inquiry |
| Gel scanning and spot picking (with Core personnel) |
per hour |
$60.35/hr |
| Coomassie staining or silver staining |
per gel |
Free |
| Cy-Dye minimum labeling (Cy2, Cy3, Cy5) |
per gel |
Inquiry |
| Sypro Ruby staining |
per gel |
$31.43 for 11-cm gel
$60.35 for 24-cm gel
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| User fee for authorized users (BioRad system 2-D gel electrophoresis
apparatus, reagents) |
per use |
IEF $40, PAGE $13/hr ($100 maximum/use) |
Please contact Core Facility for special services
Non-LSUHSC Cost
The services and costs for Researchers not affiliated with LSUHSC:
| Sample Processing |
Charging Unit |
Cost |
| Automated in-gel trypsin digest (gel slice subjected to destaining and
in gel digestion) |
per sample or per spot, whichever is applicable |
First 20 samples-$27.42 per spot, $20.49 for additional spot |
| ZipTip sample cleanup |
per sample |
$23.88 |
MALDI (4700)
| Sample Processing |
Charging Unit |
Cost |
| Simple mass spectral analysis, molecular weight information (linear
or reflector mode, peak list and spectrum, not for protein identification)
|
per sample |
First 20 samples-$53.49/sample, $34.68 for additional samples |
| Advanced automated reflector spectral analysis with database search
(MS, MS/MS of up to 5 peptide fragments per sample and database search/protein
identification) |
per sample |
First 20 samples-$64.99 per sample, $37.26 for additional sample |
| Additional MS/MS analyses after 5 fragments |
per peptide fragment ion |
$8.55/fragment |
| de novo/ProBlast search of MS/MS of a peptide (manual) |
per sample |
$85.22 |
| Experiments performed on the archiveable single-use sample plate |
additional charge on per plate |
$54.49 |
| Additional data analyses (spectral analysis, database search,
etc.) |
per hour |
$100.40 |
2-D gel electrophoresis
| Sample Processing |
Charging Unit |
Cost |
| Experiment performed by the Core Facility personnel on a Bio-Rad system
(IEF, PAGE, staining and scan; best with silver and coomassie staining
methods; fluorescence stain/CyDye is extra) |
per gel |
11-cm gel $110.78/gel, 18-cm gel $164.65/gel, Inquire for other sizes |
| Training fee for new users (PDQuest scanning and imaging analysis).
Free for authorized users. |
per hour |
$85.52/hr |
| Experiment performed by the Core Facility personnel on an Amersham Ettan
system (IEF, PAGE, staining and scan; best for fluorescence visualization
methods; fluorescence stain/CyDye is extra) |
per gel |
Inquiry |
| Gel scanning and spot picking (with Core personnel) |
per hour |
$85.52/hr |
| Coomassie staining or silver staining |
per gel |
Free |
| Cy-Dye minimum labeling (Cy2, Cy3, Cy5) |
per gel |
Inquiry |
| Sypro Ruby staining |
per gel |
$44.53 for 11-cm gel, $57.01 for 24-cm gel
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| User fee for authorized users (BioRad system 2-D gel electrophoresis
apparatus, reagents) |
per use |
IEF $45, PAGE $15/hr ($100 maximum/use) |
Please contact Core Facility for special services
The Proteomics Core Facility is located in the Clinical Science Research Building room 331. For further information please contact
Chau-Wen Chou, PhD (cchou@lsuhsc.edu).
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