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Degrees |
PhD - 1979 |
Research Interests |
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Teaching Activities |
Principles of Pharmacology |
Selected Publications |
Reed, J.R., Kelley, R.W., and Backes, W.L. (2006) An Evaluation of Methods for the Reconstitution of Cytochromes P450 and NADPH P450 Reductase into Lipid Vesicles. Drug Metab. Dispos. 34, 660-666, (PMCID: PMC2041853). Cormier, S.A., Lomnicki, S., Backes, W., and Dellinger, B. (2006) Origin and Health Impacts of Emissions of Toxic By-Products and Fine Particles from Combustion and Thermal Treatment of Hazardous Wastes and Materials. Environmental Health Persp. 114, 1-10 (PMCID: PMC1480527). Kelley, R.W., Cheng, D., and Backes, W.L. (2006) Heteromeric Complex Formation Between CYP2E1 and CYP1A2: Evidence for the Involvement of Electrostatic Interactions. Biochemistry 45, 15807-15816, (PMCID: PMC1994092). Cheng, D., Reed, J.R. Harris, D., and Backes, W.L. (2007) Inhibition of CYP2B4 by the Mechanism-based inhibitor 2-Ethynylnaphthalene: Inhibitory Potential of 2EN is Dependent on Substrate Size. Arch. Biochem. Biophys. 462, 28-37, (PMCID: PMC2041879). Cheng, D., Harris, D., Reed, J.R. and Backes, W.L. (2007) Inhibition of CYP2B4 by the Mechanism-based inhibitor 2-Ethynylnaphthalene: Evidence for the Co-Binding of Substrate and Inhibitor within the Active Site. Arch. Biochem. Biophys. 468, 174-182, (PMCID: PMC2121586). Huber III, W.J. and Backes, W.L. (2007) Expression and Characterization of Full-Length Human Heme Oxygenase-1: Presence of intact membrane-binding region leads to increased binding affinity for NADPH-cytochrome P450 reductase. Biochemistry 46, 12212-12219 (PMCID: PMC2788297). Huber III, W.J. and Backes, W.L. (2008) Quantitation of Heme Oxygenase-1: Heme Titration Increases Yield of Purified Protein, Anal. Biochem. 373, 167-169, (PMCID: PMC2186295). Reed, J.R., Brignac-Huber, L.M., Backes, W.L. (2008) Physical Incorporation of NADPH-cytochrome P450 Reductase and Cytochrome P450 into Phospholipid Vesicles using Glycocholate and Biobeads, Drug Metab. Dispos. 36, 582–588 (PMCID: PMC2789009). Huber III, W.J., Scruggs, B., and Backes, W.L. (2009) C-Terminal Membrane Spanning Region of Human Heme Oxygenase-1 Mediates a Time Dependent Complex Formation with Cytochrome P450 Reductase, Biochemistry 41, 190-197 (PMCID: PMC2789667). Huber III, W.J., Marohnic, C.C., Peters, M., Alam, J., Masters, B.S.S., and Backes, W.L. (2009) Measurement of Membrane-Bound Human Heme Oxygenase-1 Activity Using a Chemically Defined Assay System, Drug Metab. Dispos. 37, 857-864 (PMCID: PMC2680543). Reed, J.R., Eyer, M., and Backes, W.L. (2010) Functional Interactions between Cytochromes P450 1A2 and 2B4 Require Both Enzymes to Reside in the Same Phospholipid Vesicle: Evidence for physical complex formation, J. Biol. Chem. 285, 8942-8952 (PMCID: PMC2838316). Reed, J.R., Huber, W.J., and Backes, W.L. (2010) Human Heme Oxygenase-1 Efficiently Catabolizes Heme in the Absence of Biliverdin Reductase, Drug Metab. Dispos. 38, 2060-2066 (PMCID: PMC2967390). Reed, J.R., Cawley, G.F., and Backes, W.L. (2011) Assessing an Antioxidant Role for HO-1 in the Inhibition of P450-Mediated Production of Reactive Oxygen Species in Rat Liver Microsomes, Drug Metabolism Letters, 5, 6-16. Brignac-Huber, L., Reed, J.R., and Backes, W.L. (2011) Organization of NADPH-Cytochrome P450 Reductase and CYP1A2 in the Endoplasmic Reticulum – Microdomain localization affects monooxygenase function, Mol. Pharmacol. 79, 549-557. Marohnic, C.C., Huber, W.J. III, Connick, J.P., Reed, J.R., McCammon, K., Panda, S.P., Martásek, P., Backes, W.L., and Masters, B.S.S. (2011) Mutations of Human Cytochrome P450 Reductase Differentially Modulate Heme Oxygenase-I Activity and Oligomerization, Arch. Biochem. Biophys. (in press). |
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