Cover figures from the Lazartigues' lab

Breeding strategy to generate triple transgenic SARA mice overexpressing human angiotensionogen, renin and angiotensin converting enzyme type 2 in the central nervous system; bottom left: immunostaining for the Mas receptor in the paraventricular nucleus of a syn-hACE2 transgenic mouse overexpressing human ACE2 specifically in neurons; bottom right: spontaneous baroreflex sensitivity in wildtype (left), chronically hypertensive R+A+ double transgenic mice (middle) and triple transgenic SARA mice (right).

The colocalization of the human Angiotensin Converting Enzyme type 2 (ACE2; red) and mouse Microtubule-Associated Protein-2 (MAP2; green), a marker for neurons and 4”,6-diamidino-2-phenylindole (DAPI; blue), a nucleic acid marker, in the syn-hACE2 transgenic mouse rostral ventrolateral medulla (RVLM) detected by immunofluorescence. Overlapping fluorescence (yellow) indicates that the human ACE2 transgene expression is specifically targeted to neurons. Magnification: 60X.

Human angiotensin-converting enzyme 2 (hACE2) expression in neurons of rostral ventrolateral medulla (RVLM) in triple transgenic model (SARA) mice. A, Immunostaining for hACE2 (red), microtubule associated protein-2 (MAP-2; neuronal marker, green), and 4′,6-diamidino-2-phenylindole (DAPI; nucleic acid marker, blue) in the RVLM of a SARA mouse (magnification ×10). B, Increased magnification (×40) shows the presence of hACE2 in cells of the RVLM. C, MAP-2 immunofluorescence shows the distribution of neurons in the RVLM (×40). D, Overlapping fluorescence (yellow) confirms the presence of hACE2 on neurons in the RVLM (×40).

 

Lazartigues Laboratory
2015 9:58:36 AM

From Gene to Function: the Role of the Renin-Angiotensin System in the Regulation of Cardiovascular Function .

 

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