Proteomics & Metabolomics Core
The Proteomics and Metabolomics Core is dedicated to develop, implement and provide high-quality analytical methodology for determination of molecules involved in disease development, progression and treatment. The standardized techniques offered by Core generate data that can be comparable and cross referenced among specific experiments of individual investigators. Particular attention in the Core is given to quality control. All the methods in the Core are defined by a series of steps to maximize quality of the output and described in Standard Operational Procedures (SOP). Reproducibility is monitored and ensured within the system with procedures implemented to identify any changes and shifts in the platform during sample analyses. This helps to standardize quantitative measurements and facilitate research progress that leads to a better understanding of biological processes.
Services offered by Core Facilities include Sample extraction, High throughput screening, Deep metabolomics and proteomics profiling using LC- and GC-MS techniques, Targeted analysis, Data analysis, Pathway analysis and Flux analysis.
In addition, Molecular imaging is also available. In this case, spatial protein and metabolite distribution is determined using newly established MALDI HiPLEX-IHC technology available through Bruker and AmberGen. It allows for intact protein imaging by combining transcriptome relevant information from labelled protein expression mapping with advanced small molecule imaging from the same tissue section using MALDI Imaging. The method combines MALDI-based mass spectrometry with Immunohistochemistry (IHC) using specific antibodies labeled with a set of photocleavable mass-tags reporters. Platform is used to gain high resolution tissue-wide and single-cell inflammatory RNA and protein expression data with two-dimensional lineage and proximity information.
LCRC 611 (office)
LCRC 627, 928, 941 (labs)
CSRB 344 (Office)
CSRB 342 (Metabolomics lab)
CSRB 335 (IT Lab)
CSRB 331 (Proteomics lab)
Core facilities are well equipped with cutting edge instruments, which will allow us to identify and quantify all proteins relevant to the project. These resources will be readily available for the proposed studies. Instruments include two Thermo Fisher Q Exactive Plus Orbitrap mass spectrometers, one Fusion Tribrid Orbitrap mass spectrometer (all connected to nanoUPLC systems) and new Bruker’s timsTOF fleX Pro 2 mass spectrometer equipped with two MALDI sources and nanoElute UPLC System for global profiling. In addition, Agilent’s 6495C LCTQ MS System equipped with 1200 HPLC system for targeted analysis and Agilent’s 7250 GC/Q-TOF for polar metabolite quantification are available. Core also has bioinformatics capabilities with database search engines and additional data mining software.
Jiri Adamec, PhD
Professor
Director of Core Laboratories
LSUHSC School of Medicine
Stanley S. Scott Cancer Center
Department of Interdisciplinary Oncology
1700 Tulane Ave, Room 611
New Orleans, LA70112
P: (504) 210-3538
F: (504) 210-2970