The protocols we follow are similar to those from other Proteomics Core Facilities. The following sites can provide more information about the basics of Proteomic tools:
M.W. Keck Facility-Mass Spectrometry and Protein Chemistry Services at Yale University: http://keck.med.yale.edu/prochem/
ExPASy Proteomics Server - Expert Protein Analysis System proteomics server of the Swiss Institute of Bioinformatics (SIB): dedicated to the analysis of protein sequences and structures as well as 2-D PAGE. http://ca.expasy.org/
American Society for Mass Spectrometry: http://www.asms.org
An Introduction of Mass Spectrometry - “What is mass spectrometry”: http://www.asms.org/whatisms/index.html
Review paper “Protein identification methods in Proteomics”: Kris Gevaer, et al.; Electrophoresis, 2000, 21, 1145-1154.
"Mass Spectrometry-Based Protemoics": Ruedi Aebersold and Matthias Mann; Nature, 2003, 422, 198-207.
Mascot database search: David N. Perkins et al.; Electrophoresis, 1999, 20, 3551-3567.
The Proteomics Core Facility is located in the Clinical Science Research Building room 331.