Neuroscience Center of Excellence
School of Medicine, LSU Health
2020 Gravier Street
Room 935A
New Orleans, LA 70112

Phone: (504) 599-0849

Fax: (504) 599-0488

lbelay@lsuhsc.edu

Key to dialogue between brain cells to protect against stroke
Dr. Nicolas G. Bazan and Dr. Ludmila Belayev
Date: August 6, 2020
Source: Louisiana State University Health Sciences Center
Summary: LSU Health New Orleans research has unlocked a key fundamental mechanism in the communication between brain cells when confronted with stroke and found DHA not only protected neuronal cells and promoted their survival, but also helped maintain their integrity and stability.

Degrees

Medical University School, Novosibirsk, Russia, Neurosurgery, 1978-1982.

Medical University School, Novosibirsk, Russia, Surgery, 1977-1978.

Medical University School, Novosibirsk, Russia, M.D., 1970-1976.

Post-Doctoral Fellowships: Cerebral Vascular Disease Research Center, Department of Neurology, University of Miami School of Medicine, 1994-1996.

Bio

2013 to present: Professor of Neurology, School of Medicine, LSUHSC

2012 to present: Professor of Neurosurgery and Neuroscience, School of Medicine, LSUHSC

2006 to 2012: Research Associate Professor of Neurosurgery and Neuroscience, School of Medicine, LSUHSC

2005 to 2006: Director of Commercial Research and Pharmacology, Inotek Pharmaceuticals Corporation

2003-2005: Research Associate Professor, Department of Neurology, University of Miami School of Medicine

1998-2003: Research Assistant Professor, Department of Neurology, University of Miami School of Medicine

1994-1998: Postdoctoral Research Associate, Department of Neurology, University of Miami School of Medicine

1992-1994: Neuroscientist, Department of Pharmacology, Pharmos Corp., Rehovot, Israel

1979-1990: Neurosurgeon, Department of Neurosurgery, Novosibirsk Regional Hospital, Russia

1989: Neurovascular Fellowship-Institute of Neurosurgery N.N. Burdenko, Moscow, Russia

1991: Course of Internal Medicine, Ishilov Hospital, Tel-Aviv, Israel

CURRENT RESEARCH PROJECTS
Neuroprotection with Docosanoids in focal cerebral ischemia in rats and mice.

Development and evaluations of experimental traumatic brain injury models in rats.

Development and evaluations of experimental models of glioblastoma in mice.

Research Interests

I. Development and evaluation of animal models of stroke:

Models of global cerebral ischemia in rats and mice:
a) Two-vessel occlusion model of forebrain ischemia
b) Four-vessel occlusion model of forebrain ischemia

Models of focal cerebral ischemia in rats and mice:
a) Middle cerebral artery occlusion by intraluminal suture
b) Mechanical (electrocoagulation, microclip)

Hemorrhagic models in rats and mice:
a) Intracerebral hemorrhagic models (blood, collagenase)
b) SAH and vasospasm: (injection of blood into cisterna magna and puncture of cerebral artery)

II. Development and evaluation of animal models of traumatic brain injury:
a) Lateral fluid percussion injury model
b) Controlled weight drop brain injury

III. Recovering function after brain injury (neural plasticity and reorganization), expertise in different neurobehavioral tests.

IV. Evaluation of blood-brain integrity.

V. Histopathology and image analysis of the brain after stroke and head trauma.

VI. Neuroprotection in brain ischemia and traumatic brain injury.

VII. Non-clinical animal pharmacology, pharmacokinetics and toxicology studies.

Committees & Administrative Responsibilities

Committees & Administrative Responsibilities

Peer Review Study committees:

Reviewer, NIH, BINP, Washington, DC, June 17-18, 2019.

Reviewer, NIH, ZNS1 SRB-D (03) Washington, DC, March 18-19, 2019.

Reviewer, NIH, ZRG1 BDCN-Y (02) Special Emphasis Panel, March 5, 2019.

Chair, NIH, ZRG1 BDCN-Y (02) Special Emphasis Panel, October 24, 2018.

Reviewer, NIH, ZRG1 BDCN-Y (02) Special Emphasis Panel, February 28, 2018.

Reviewer, NIH, ZRG1 BDCN-K (02) Special Emphasis Panel, October 26, 2017.

Reviewer, NIH, ZRG1 BDCN-K (02) Special Emphasis Panel, June 14, 2017.

Reviewer, NIH, ZRG1 BDCN-K (02) Special Emphasis Panel, October 26, 2016.

Reviewer, NIH, ZRG1 BDCN-K (02) Special Emphasis Panel, June 17, 2016.

Reviewer, NIH, BINP study section, Chicago, IL, June 9-10, 2016.

Reviewer, NIH, ZRG1 IFCN-Z (04) Special Emphasis Panel, April 20, 2016.

Reviewer, NIH, ZRG1 BDCN-K (02) Special Emphasis Panel, March 2, 2016.

Editorial Board:

Journal of Neurology & Translational Neuroscience (2013 –present)
BMC Neurology (2003–present)
ISRN Neurology (2010-present)
The Science Advisory Board (2010-present)
Experimental & Translational Stroke Medicine (2012 – 2016)
Stroke (2005-2007)

Selected Publications

Selected Publications

Journal publications:

Belayev L, Hong SH, Menghani H, Marcell SJ, Obenaus A, Freitas RS, Khoutorova L, Balaszczuk V, Jun B, Oriá RB, Bazan NG. Docosanoids promote neurogenesis and angiogenesis, blood-brain barrier integrity, penumbra protection, and neurobehavioral recovery after experimental ischemic stroke.Mol Neurobiol. 55:7090-7106, 2018. PMID: 29858774.
Bhattacharjee S, B. Jun, L. Belayev, J. Heap, M.A. Kautzmann, A. Obenaus, H. Menghani, S.J. Marcell, L. Khoutorova, R. Yang, N.A. Petasis, N.G. Bazan. Elovanoids are a novel class of homeostatic lipid mediators that protect neural cell integrity upon injury. Science Advances, 3:e1700735, 2017.
Belayev L, Mukherjee P, Balaszczuk V, Obenaus A, Khoutorova L, Hong SH, and Calandria J, Bazan NG. Neuroprotectin D1 upregulates Iduna expression and provides protection in cellular uncompensated oxidative stress and in experimental ischemic stroke. Cell Death and Differentiation, 6, 1091-1099, 2017. PMID: 28430183.
Musto A, Rosencrans R, Walker C, Bhattacharjee S, Raulji C, Belayev L, Fang Z, Gordon WC, Bazan NG. Dysfunctional epileptic neuronal circuits and dysmorphic dendritic spines are mitigated by platelet-activating factor receptor antagonism. Scientific Reports/Nature, 6:30298, 2016. PMID: 27444269.
Belayev L, Khoutorova L, Hong SH, Obenaus A. A novel therapeutic strategy for experimental stroke using docosahexaenoic acid complexed to human albumin. Oilseeds and fats, Crops and Lipids, 23:1-7, 2016.
Rossi JL, Todd T, Daniels Z, Bazan NG, Belayev L. Interferon Stimulated Gene 15 upregulation precedes the development of blood brain barrier disruption and cerebral edema after traumatic brain injury in young mice. J Neurotrauma. 32:1101-1108, 2015 PMID: 25669448.
Calandria JM, Asatryan A, Balaszczuk V, Knott EJ, Mukherjee PK, Belayev L, Bazan NG. NPD1-mediated stereoselective regulation of BIRC3 expression through cREL is decisive for neural cell survival. Cell Death and Differentiation, 1-15, 2015. PMID: 25633199.
Hong SH, Khoutorova L, Bazan NG, Belayev L. Docosahexaenoic acid protects blood-brain barrier permeability induced by focal cerebral ischemia in rats. Exp Transl Stroke Med, 7:3. 2015. PMID: 25642315
Eady TN, Khoutorova L, Obenaus A, Mohd-Yusof A, Bazan NG, Belayev L. Docosahexaenoic acid complexed to human albumin provides neuroprotection after experimental stroke in aged rats. Neurobiology of Disease, 62:1-7, 2014. PMID: 24063996.
Hong SH, Belayev L, Khoutorova L, Obenaus A, Bazan NG. Docosahexaenoic acid confers enduring neuroprotection in experimental stroke. J Neurol Sci, 338:135-141, 2014. PMID: 24433927.
Eady TN, Khoutorova L, Anzola DV, Hong SH, Obenaus A, Mohd-Yusof A, Bazan NG, Belayev L. Acute treatment with docosahexaenoic acid complexed to albumin reduces injury after a permanent focal cerebral ischemia in rats. PLoS One, 8:e77237, 2013. PMID: 24194876.
Rossi JL, Todd T, Bazan NG, Belayev L. Inhibition of myosin light chain kinase attenuates cerebral edema following traumatic brain injury in postnatal mice.J. Neurotrauma, 30:1772-1679, 2013. PMID: 23984869.
Eady TN, Belayev L, Khoutorova L, Atkins KD, Zhang C, Bazan NG. Docosahexaenoic Acid signaling modulates cell survival in experimental ischemic stroke penumbra and initiates long-term repair in young and aged rats. PLoS One, 7:e46151, 2012. PMID: 23118851.
Bazan NG, Eady TN, Khoutorova L, Atkins KD, Hong S, Lu Y, Zhang C, Jun B, Obenaus A, Fredman G, Zhu M, Winkler JW, Petasis NA, Serhan CN, Belayev L. Novel aspirin-triggered Neuroprotectin D1 attenuates cerebral ischemic injury after experimental stroke. Exp Neurol, 236:122-130, 2012. PMID: 22542947.
Eady TN, Khoutorova L, Atkins KD, Bazan NG, Belayev L. Docosahexaenoic acid complexed to human albumin in experimental stroke: neuroprotective efficacy with a wide therapeutic window.Exp Transl Stroke Med, 4:19, 2012. PMID: 22980673.
Belayev L, Eady TN, Khoutorova L, Atkins K, Obenaus A, Cordoba M, Vaquero JJ, Builla JA, Bazan NG. Superior Neuroprotective Efficacy of LAU-0901, a novel Platelet Activating Factor Antagonist, in Experimental Stroke. Translational Stroke Research, 3:154-163, 2012. PMID: 22408693.
Serhan CN, Fredman G, Yang R, Karamnov S, Belayev LS, Bazan NG, Zhu M, Winkler JW, Petasis NA. Novel Proresolving Aspirin-Triggered DHA Pathway. Cell:Chem Biol. 18, 976-987, 2011. PMID: 21867913.
Belayev L, Khoutorova L, Atkins K, Eady TN, Hong S, Lu Y, Obenaus A, Bazan NG. Docosahexaenoic acid therapy of experimental ischemic stroke. Translational Stroke Research, 2:33-41, 2011. PMID: 21423332.
Belayev L, Khoutorova L, Atkins K, Bazan NG. Robust Docosahexaenoic Acid-Mediated Neuroprotection in a Rat Model of Transient Focal Cerebral Ischemia. Stroke, 40:3121-3126, 2009. PMID: 19542051
Belayev L, Khoutorova L, Zhao KL, Davidoff AW, Moore AF, Cramer SC. A novel therapeutic strategy for experimental stroke. Brain Research, 1280:117-123, 2009.
:184-190, 2009.
Belayev L, Khoutorova L, Atkins K, Gordon WC, Bazan NG. LAU-0901, a novel platelet-activating factor antagonist, is highly neuroprotective in cerebral ischemia. Exp. Neurol, 214:253-258, 2008.

Book Chapters:

L. Belayev. Mouse Intracerebral Hemorrhage Model. In: Jun Chen, Zao Xu, Xiao-Ming Xu, and John Zhang, (Eds), Animal Models of Acute Neurological Injuries, The Springer, 2018.
L. Belayev, M. Endres and V. Prinz. Focal cerebral ischemia in the mouse and rat using the intraluminal suture-filament model. In: U. Dirnagl, (Ed), Rodent Models of Stroke, The Springer Protocols, Chapter 4, 31-43, 2016.
L Belayev, Freitas RS, Marcell SJ, Bazan NG. Lipid mediators. In: Caplan LR, Biller J, Leary MC, Lo EH, Thomas AJ, Yenari M, Zhang JH (Eds), Primer on Cerebrovascular Diseases, Elsevier, Second Edition, Chapter 52, 2016.
L. Belayev. Overcoming barriers to translation from experimental stroke models. In: Lapchak and Zhang (Eds), Stroke targets to clinical trials, Springer, Chapter 24, 471-492, 2012.
L. Belayev, Y. Lu, NG Bazan. Brain ischemia and reperfusion: cellular and molecular mechanisms in stroke injury. In: Brady, Albers, Price, Siegel (Eds), Basic Neurochemistry, Elsevier, Chapter 35, 621-642, 2012.
L. Belayev, M. Endres and V. Prinz. Focal cerebral ischemia in the mouse and rat using the intraluminal suture-filament model. In: U. Dirnagl, (Ed), Rodent Models of Stroke, The Springer, Chapter 4, 29-40, 2010.
L. Belayev. Mouse Intracerebral Hemorrhage Model. In: Jun Chen, Zao Xu, Xiao-Ming Xu, and John Zhang, (Eds), Animal Models of Acute Neurological Injuries, The Humana Press Inc, 349-357, 2009.

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